COMPRISON OF LIGHT MICROSCOPE AND ELECTRON MICROSCOPE

by • 14/07/2011 • Old Pattern Biology NotesComments (0)936

ELECTRON MICROSCOPE

This is the most advanced form of microscope. Its resolving power is 250 times or more that of a compound microscope. In this microscope, a beam of electrons under high voltage is passed through the object and its image is reflected on to a screen through an electro magnetic lens to make a photograph.

(Diagram)

With the help of microscope, any object an be magnified up to 250,000 its original size.

COMPRISON OF LIGHT MICROSCOPE AND ELECTRON MICROSCOPE

Light Microscope

  • The radiation source is light so it is called light microscope.
  • Wavelength of light is 400 – 700 nm.
  • Maximum resolution is 200 nm.
  • Maximum useful magnification is X 1500 with eye.
  • Lenses are used.

Electron Microscope

  • The radiation source is beam of electrons, so it is called Electron Microscope.
  • Wavelength of beam of electrons is 0.005 nm.
  • Maximum resolution is 0.5 nm.
  • Maximum useful magnification is X. 250,000 on screen as image or photograph.
  • Electromagnets are used.
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